Plasmid_Backbone

Part:BBa_J179000:Design

Designed by: Jara Radeck   Group: Mascher Lab   (2013-12-03)


pBS1C: Bacillus subtilis vector, amyE integration, CAM-resistance


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 6084
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 6090
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 6084
    Illegal BamHI site found at 6066
    Illegal XhoI site found at 3574
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 6084
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 6084
    Plasmid lacks a suffix.
    Illegal XbaI site found at 6099
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 4501


Design Notes

This is one of three empty vectors with compatible integration loci and resistance cassettes to allow combination of up to three constructs of choice in one single strain.


Source

pDG1731

References